Drag and drop the sample H&E Kidney sample onto the microscope stage.

MICROSCOPE MODE

SETUP & BRIGHTFIELD

FLUORESCENCE

PHASE CONTRAST

DIC

POLARISED LIGHT

REFLECTION

DARK FIELD

CONFOCAL

SUPER-RES – SMLM

INSERT

PHASE

DIC FILTER

POLARISING FILTER

DARKFIELD RING

HALOGEN LAMP

MERCURY LAMP

FOCUS

FIELD DIAPHRAGM

RIGHT EYEPIECE

OBJECTIVES

MOVE SLIDE

LIGHT INTENSITY

CONDENSER POSITIONING

CONDENSER FOCUS

CONDENSER APERTURE

LIGHT INTENSITY

CHANNEL 3
CHANNEL 4

MICROSCOPE MODE

SETUP & BRIGHTFIELD

FLUORESCENCE

PHASE CONTRAST

DIC

POLARISED LIGHT

REFLECTION

DARK FIELD

CONFOCAL

SUPER-RES – SMLM

CHANNEL 2

Ex 488 Em 500-550

100%

80V

60

0%

20V

-60

LASER

GAIN (HV)

OFFSET

CHANNEL 3

Ex 561 Em 570-600

100%

80V

60

0%

20V

-60

LASER

GAIN (HV)

OFFSET

CHANNEL 4

Ex 640 Em 650-700

100%

80V

60

0%

20V

-60

LASER

GAIN (HV)

OFFSET

Z-STACK & PINHOLE

TOP Z-POS

BOTTOM
Z-POS

no set

no set

OPTIMISED

RANGE

OPTICAL SECTION THICKNESS

LIVE VIEW

CAPTURE IMAGE

CAPTURE Z-STACK

SCAN RATE (FRAMES/SEC)

0.5

ZOOM

CHANNEL 1

MICROSCOPE MODE

SETUP & BRIGHTFIELD

FLUORESCENCE

PHASE CONTRAST

DIC

POLARISED LIGHT

REFLECTION

DARK FIELD

CONFOCAL

SUPER-RES – SMLM

CHANNEL 1

Ex 647 Em 665-715

LASER

1000 µm

FOCUS

CAMERA SETTINGS

DATA ACQUISITION

ACTIVATION LASER

POWER

2%

DURATION

100

MS

EVERY

500

FRAMES

LIVE VIEW

CAPTURE IMAGE

CAPTURE SMLM IMAGE

DATA PROCESSING

INTENSITY THRESHOLD

LOW

HIGH

PROCESS SMLM IMAGE

DRIFT CORRECTION

IMAGING SOFTWARE

×

EXPOSURE TIME

0ms

8ms

HISTOGRAM

×

HISTOGRAM

×

×

ACTIVITIES MENU

VISUALISING YOUR SAMPLE

OBJECTIVE LENS

PINHOLE

PIXEL DWELL

PIXEL ARRAYS

MULTICHANNEL IMAGES

3D & Z-STACKS

Excitation–emission: example for viewing GFP

Click to see each channel and then combine the images into the overlay.

Click to see each channel and then combine the images into the overlay.

PINHOLE = 1.0 AU
PINHOLE = OPEN
PINHOLE = 0.4 AU (closed)
PINHOLE = OPEN
PINHOLE = 1.0 AU
12 µsec
2 µsec
4 µsec
CHANNEL 4

×

×

×

Please wait while the image scans

×